Application of a multiplex polymerase chain reaction (mPCR) assay to detect fraud by substitution of bovine meat cuts with water buffalo meat in Northern Brazil
نویسندگان
چکیده
منابع مشابه
Identification of Listeria Spp. Strains Isolated from Meat Products and Meat Production Plants by Multiplex Polymerase Chain Reaction
Listeriosis is a foodborne disease caused by Listeria monocytogenes and is considered as a serious health problem, due to the severity of symptoms and the high mortality rate. Recently, other Listeria species have been associated with disease in human and animals. The aim of this study was to develop a multiplex polymerase chain reaction (PCR) in order to simultaneously detect six Listeria spec...
متن کاملDetection of Chicken Meat Adulteration in Raw Hamburger Using Polymerase Chain Reaction
Background: Detection of food adulteration is an important issue from aspects of food control and food regulation. This study aimed to detect adulteration of chicken meat in raw hamburger using species specific Polymerase Chain Reaction (PCR). Methods: Raw hamburgers including 42 handmade and 48 industrial samples were collected from 90 restaurants and supermarkets. Following genomic DNA extra...
متن کاملIdentification of Meat Species by Polymerase Chain Reaction ( PCR ) Technique
The origin of horse, dog, cat, bovine, sheep, porcine, and goat meat was determined by the polymerase chain reaction (PCR) technique, using species-specific primers. Test mixtures of meat were prepared by adding 5%, 2.5%, 1%, 0.5%, and 0.1% levels of pork, horse, cat, or dog meat to beef, sheep, and goat meat. Samples taken from those combinations were analyzed by PCR for species determination....
متن کاملIdentification of meat species in pet foods using a real-time polymerase chain reaction (PCR) assay
Product mislabeling, adulteration, and substitution are increasing concerns in highly processed foods, including pet foods. Although regulations exist for pet foods, there is currently a lack of information on the prevalence of pet food mislabeling. The objective of this study was to perform a market survey of pet foods and pet treats marketed for domestic canines and felines to identify meat s...
متن کاملDevelopment and evaluation of a polymerase chain reaction assay to detect Bovine herpesvirus 1
A polymerase chain reaction (PCR) assay to detect Bovine herpesvirus 1 (BoHV-1) has been developed and evaluated. This assay was based on the amplification of a viral thymidine kinase (tk) gene fragment, for which a novel pair of primers and different PCR conditions were tested. Under optimal conditions, no amplification was observed from heterologous herpesviruses or other bovine viruses. Howe...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: CyTA - Journal of Food
سال: 2019
ISSN: 1947-6337,1947-6345
DOI: 10.1080/19476337.2019.1650832